Thin-layer chromatographic identification of Chinese propolis using chemometric fingerprinting.
Identifieur interne : 001F34 ( Main/Exploration ); précédent : 001F33; suivant : 001F35Thin-layer chromatographic identification of Chinese propolis using chemometric fingerprinting.
Auteurs : Tie-Xin Tang ; Wei-Yan Guo ; Ye Xu ; Si-Ming Zhang ; Xin-Jun Xu ; Dong-Mei Wang ; Zhi-Min Zhao ; Long-Ping Zhu ; De-Po YangSource :
- Phytochemical analysis : PCA [ 1099-1565 ]
Descripteurs français
- KwdFr :
- Abeilles (MeSH), Analyse de regroupements (MeSH), Animaux (MeSH), Arbres (MeSH), Cartographie peptidique (MeSH), Chromatographie sur couche mince (méthodes), Populus (composition chimique), Propolis (composition chimique), Propolis (isolement et purification), Résines végétales (composition chimique), Résines végétales (isolement et purification).
- MESH :
- composition chimique : Populus, Propolis, Résines végétales.
- isolement et purification : Propolis, Résines végétales.
- méthodes : Chromatographie sur couche mince.
- Abeilles, Analyse de regroupements, Animaux, Arbres, Cartographie peptidique.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Propolis, Resins, Plant.
- chemistry : Populus.
- chemical , isolation & purification : Propolis, Resins, Plant.
- methods : Chromatography, Thin Layer.
- Animals, Bees, Cluster Analysis, Peptide Mapping, Trees.
Abstract
INTRODUCTION
Poplar tree gum has a similar chemical composition and appearance to Chinese propolis (bee glue) and has been widely used as a counterfeit propolis because Chinese propolis is typically the poplar-type propolis, the chemical composition of which is determined mainly by the resin of poplar trees. The discrimination of Chinese propolis from poplar tree gum is a challenging task.
OBJECTIVE
To develop a rapid thin-layer chromatographic (TLC) identification method using chemometric fingerprinting to discriminate Chinese propolis from poplar tree gum.
METHODS
A new TLC method using a combination of ammonia and hydrogen peroxide vapours as the visualisation reagent was developed to characterise the chemical profile of Chinese propolis. Three separate people performed TLC on eight Chinese propolis samples and three poplar tree gum samples of varying origins. Five chemometric methods, including similarity analysis, hierarchical clustering, k-means clustering, neural network and support vector machine, were compared for use in classifying the samples based on their densitograms obtained from the TLC chromatograms via image analysis.
RESULTS
Hierarchical clustering, neural network and support vector machine analyses achieved a correct classification rate of 100% in classifying the samples. A strategy for TLC identification of Chinese propolis using chemometric fingerprinting was proposed and it provided accurate sample classification.
CONCLUSION
The study has shown that the TLC identification method using chemometric fingerprinting is a rapid, low-cost method for the discrimination of Chinese propolis from poplar tree gum and may be used for the quality control of Chinese propolis.
DOI: 10.1002/pca.2502
PubMed: 24497376
Affiliations:
Links toward previous steps (curation, corpus...)
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Xu</name></author><author><name sortKey="Wang, Dong Mei" sort="Wang, Dong Mei" uniqKey="Wang D" first="Dong-Mei" last="Wang">Dong-Mei Wang</name></author><author><name sortKey="Zhao, Zhi Min" sort="Zhao, Zhi Min" uniqKey="Zhao Z" first="Zhi-Min" last="Zhao">Zhi-Min Zhao</name></author><author><name sortKey="Zhu, Long Ping" sort="Zhu, Long Ping" uniqKey="Zhu L" first="Long-Ping" last="Zhu">Long-Ping Zhu</name></author><author><name sortKey="Yang, De Po" sort="Yang, De Po" uniqKey="Yang D" first="De-Po" last="Yang">De-Po Yang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014 May-Jun</date><idno type="RBID">pubmed:24497376</idno><idno type="pmid">24497376</idno><idno type="doi">10.1002/pca.2502</idno><idno type="wicri:Area/Main/Corpus">002311</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002311</idno><idno type="wicri:Area/Main/Curation">002311</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002311</idno><idno type="wicri:Area/Main/Exploration">002311</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Thin-layer chromatographic identification of Chinese propolis using chemometric fingerprinting.</title><author><name sortKey="Tang, Tie Xin" sort="Tang, Tie Xin" uniqKey="Tang T" first="Tie-Xin" last="Tang">Tie-Xin Tang</name><affiliation><nlm:affiliation>School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China, 510006.</nlm:affiliation><wicri:noCountry code="subField">510006</wicri:noCountry></affiliation></author><author><name sortKey="Guo, Wei Yan" sort="Guo, Wei Yan" uniqKey="Guo W" first="Wei-Yan" last="Guo">Wei-Yan Guo</name></author><author><name sortKey="Xu, Ye" sort="Xu, Ye" uniqKey="Xu Y" first="Ye" last="Xu">Ye Xu</name></author><author><name sortKey="Zhang, Si Ming" sort="Zhang, Si Ming" uniqKey="Zhang S" first="Si-Ming" last="Zhang">Si-Ming Zhang</name></author><author><name sortKey="Xu, Xin Jun" sort="Xu, Xin Jun" uniqKey="Xu X" first="Xin-Jun" last="Xu">Xin-Jun Xu</name></author><author><name sortKey="Wang, Dong Mei" sort="Wang, Dong Mei" uniqKey="Wang D" first="Dong-Mei" last="Wang">Dong-Mei Wang</name></author><author><name sortKey="Zhao, Zhi Min" sort="Zhao, Zhi Min" uniqKey="Zhao Z" first="Zhi-Min" last="Zhao">Zhi-Min Zhao</name></author><author><name sortKey="Zhu, Long Ping" sort="Zhu, Long Ping" uniqKey="Zhu L" first="Long-Ping" last="Zhu">Long-Ping Zhu</name></author><author><name sortKey="Yang, De Po" sort="Yang, De Po" uniqKey="Yang D" first="De-Po" last="Yang">De-Po Yang</name></author></analytic><series><title level="j">Phytochemical analysis : PCA</title><idno type="eISSN">1099-1565</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Bees (MeSH)</term><term>Chromatography, Thin Layer (methods)</term><term>Cluster Analysis (MeSH)</term><term>Peptide Mapping (MeSH)</term><term>Populus (chemistry)</term><term>Propolis (chemistry)</term><term>Propolis (isolation & purification)</term><term>Resins, Plant (chemistry)</term><term>Resins, Plant (isolation & purification)</term><term>Trees (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Abeilles (MeSH)</term><term>Analyse de regroupements (MeSH)</term><term>Animaux (MeSH)</term><term>Arbres (MeSH)</term><term>Cartographie peptidique (MeSH)</term><term>Chromatographie sur couche mince (méthodes)</term><term>Populus (composition chimique)</term><term>Propolis (composition chimique)</term><term>Propolis (isolement et purification)</term><term>Résines végétales (composition chimique)</term><term>Résines végétales (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Propolis</term><term>Resins, Plant</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Populus</term><term>Propolis</term><term>Résines végétales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Propolis</term><term>Resins, Plant</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Propolis</term><term>Résines végétales</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Thin Layer</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Chromatographie sur couche mince</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Bees</term><term>Cluster Analysis</term><term>Peptide Mapping</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Abeilles</term><term>Analyse de regroupements</term><term>Animaux</term><term>Arbres</term><term>Cartographie peptidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>INTRODUCTION</b></p><p>Poplar tree gum has a similar chemical composition and appearance to Chinese propolis (bee glue) and has been widely used as a counterfeit propolis because Chinese propolis is typically the poplar-type propolis, the chemical composition of which is determined mainly by the resin of poplar trees. The discrimination of Chinese propolis from poplar tree gum is a challenging task.</p></div><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>To develop a rapid thin-layer chromatographic (TLC) identification method using chemometric fingerprinting to discriminate Chinese propolis from poplar tree gum.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>A new TLC method using a combination of ammonia and hydrogen peroxide vapours as the visualisation reagent was developed to characterise the chemical profile of Chinese propolis. Three separate people performed TLC on eight Chinese propolis samples and three poplar tree gum samples of varying origins. Five chemometric methods, including similarity analysis, hierarchical clustering, k-means clustering, neural network and support vector machine, were compared for use in classifying the samples based on their densitograms obtained from the TLC chromatograms via image analysis.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Hierarchical clustering, neural network and support vector machine analyses achieved a correct classification rate of 100% in classifying the samples. A strategy for TLC identification of Chinese propolis using chemometric fingerprinting was proposed and it provided accurate sample classification.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>The study has shown that the TLC identification method using chemometric fingerprinting is a rapid, low-cost method for the discrimination of Chinese propolis from poplar tree gum and may be used for the quality control of Chinese propolis.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24497376</PMID><DateCompleted><Year>2014</Year><Month>12</Month><Day>15</Day></DateCompleted><DateRevised><Year>2014</Year><Month>04</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1099-1565</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>3</Issue><PubDate><MedlineDate>2014 May-Jun</MedlineDate></PubDate></JournalIssue><Title>Phytochemical analysis : PCA</Title><ISOAbbreviation>Phytochem Anal</ISOAbbreviation></Journal><ArticleTitle>Thin-layer chromatographic identification of Chinese propolis using chemometric fingerprinting.</ArticleTitle><Pagination><MedlinePgn>266-72</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/pca.2502</ELocationID><Abstract><AbstractText Label="INTRODUCTION" NlmCategory="BACKGROUND">Poplar tree gum has a similar chemical composition and appearance to Chinese propolis (bee glue) and has been widely used as a counterfeit propolis because Chinese propolis is typically the poplar-type propolis, the chemical composition of which is determined mainly by the resin of poplar trees. The discrimination of Chinese propolis from poplar tree gum is a challenging task.</AbstractText><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To develop a rapid thin-layer chromatographic (TLC) identification method using chemometric fingerprinting to discriminate Chinese propolis from poplar tree gum.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">A new TLC method using a combination of ammonia and hydrogen peroxide vapours as the visualisation reagent was developed to characterise the chemical profile of Chinese propolis. Three separate people performed TLC on eight Chinese propolis samples and three poplar tree gum samples of varying origins. Five chemometric methods, including similarity analysis, hierarchical clustering, k-means clustering, neural network and support vector machine, were compared for use in classifying the samples based on their densitograms obtained from the TLC chromatograms via image analysis.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Hierarchical clustering, neural network and support vector machine analyses achieved a correct classification rate of 100% in classifying the samples. A strategy for TLC identification of Chinese propolis using chemometric fingerprinting was proposed and it provided accurate sample classification.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The study has shown that the TLC identification method using chemometric fingerprinting is a rapid, low-cost method for the discrimination of Chinese propolis from poplar tree gum and may be used for the quality control of Chinese propolis.</AbstractText><CopyrightInformation>Copyright © 2014 John Wiley & Sons, Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Tie-xin</ForeName><Initials>TX</Initials><AffiliationInfo><Affiliation>School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China, 510006.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Wei-yan</ForeName><Initials>WY</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Ye</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Si-ming</ForeName><Initials>SM</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Xin-jun</ForeName><Initials>XJ</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Dong-mei</ForeName><Initials>DM</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Zhi-min</ForeName><Initials>ZM</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Long-ping</ForeName><Initials>LP</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>De-po</ForeName><Initials>DP</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>02</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochem Anal</MedlineTA><NlmUniqueID>9200492</NlmUniqueID><ISSNLinking>0958-0344</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012116">Resins, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>9009-62-5</RegistryNumber><NameOfSubstance UI="D011429">Propolis</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001516" MajorTopicYN="N">Bees</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002855" MajorTopicYN="N">Chromatography, Thin Layer</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016000" MajorTopicYN="N">Cluster Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010449" MajorTopicYN="N">Peptide Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011429" MajorTopicYN="N">Propolis</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012116" MajorTopicYN="N">Resins, Plant</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Chinese propolis</Keyword><Keyword MajorTopicYN="N">chemometrics</Keyword><Keyword MajorTopicYN="N">fingerprinting</Keyword><Keyword MajorTopicYN="N">identification</Keyword><Keyword MajorTopicYN="N">image analysis</Keyword><Keyword MajorTopicYN="N">pattern recognition</Keyword><Keyword MajorTopicYN="N">thin-layer chromatography</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>08</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>11</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>11</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>2</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>2</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24497376</ArticleId><ArticleId IdType="doi">10.1002/pca.2502</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Guo, Wei Yan" sort="Guo, Wei Yan" uniqKey="Guo W" first="Wei-Yan" last="Guo">Wei-Yan Guo</name><name sortKey="Tang, Tie Xin" sort="Tang, Tie Xin" uniqKey="Tang T" first="Tie-Xin" last="Tang">Tie-Xin Tang</name><name sortKey="Wang, Dong Mei" sort="Wang, Dong Mei" uniqKey="Wang D" first="Dong-Mei" last="Wang">Dong-Mei Wang</name><name sortKey="Xu, Xin Jun" sort="Xu, Xin Jun" uniqKey="Xu X" first="Xin-Jun" last="Xu">Xin-Jun Xu</name><name sortKey="Xu, Ye" sort="Xu, Ye" uniqKey="Xu Y" first="Ye" last="Xu">Ye Xu</name><name sortKey="Yang, De Po" sort="Yang, De Po" uniqKey="Yang D" first="De-Po" last="Yang">De-Po Yang</name><name sortKey="Zhang, Si Ming" sort="Zhang, Si Ming" uniqKey="Zhang S" first="Si-Ming" last="Zhang">Si-Ming Zhang</name><name sortKey="Zhao, Zhi Min" sort="Zhao, Zhi Min" uniqKey="Zhao Z" first="Zhi-Min" last="Zhao">Zhi-Min Zhao</name><name sortKey="Zhu, Long Ping" sort="Zhu, Long Ping" uniqKey="Zhu L" first="Long-Ping" last="Zhu">Long-Ping Zhu</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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